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Oligos Etc
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Promega
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Image Search Results
Journal: RNA
Article Title: Adaptive evolution of testis-specific, recently evolved, clustered miRNAs in Drosophila
doi: 10.1261/rna.044644.114
Figure Lengend Snippet: Proof of principle for adaptive regulatory activity of newly evolved, testis-restricted miRNAs. Luciferase sensor assay tests for binding and regulatory potential of two seed-divergent, testes-specific, recently evolved miRNAs: ( A ) mir-2498 and ( B ) mir-303 . ( Top ) miR homologs with three seed mismatches were assayed for regulatory potential to congruent sensor targets. ( Bottom ) In all cases, miRs significantly repressed their cognate targets but not noncognate ones. This test validates that divergent miRs of the same family (1) have regulatory potential for gene regulation and (2) base-identity constraint to repress only a set of specific-specific target genes.
Article Snippet: Renilla luciferase sensors for D. melanogaster miR-2498, D. melanogaster miR-303, D. erecta miR-2498, and
Techniques: Activity Assay, Luciferase, Binding Assay